Research:
Increasing Agrobacterium-mediated transformation frequency in rice (Japonicum cv. Nipponbare)
A: Type II callus emerging from mature rice seed
PTF has been working on methods to establish an efficient Agrobacterium-mediated rice transformation system using mature seed derived callus. Current efforts to improve and expand upon the standard transformation protocol include: various comparison trials involving the composition of co-cultivation media, using different selection agents and exploring a range of tissue culture incubation and growth chamber environmental conditions. Transformation and tissue culture work is also being done with different Japonicum cultivars, Agrobacterium strains and vectors in order to provide researchers with an efficient, yet flexible, rice transformation pipeline.
Multiple plantlets regenerating from single transgenic rice event
