Spring workshop on plant transformation
GDCB 542D (1 credit)
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This is a one-credit laboratory course aiming at learning basic knowledge and technique in plant genetic transformation and molecular analysis of transgenic plants.
Who should take this course:
- Graduate students majoring in any discipline of plant sciences.
- Graduate students majoring in any science discipline with an intention to collaborate with plant scientists.
- Anyone interested in plant genetic transformation
| 1.Make plant media | 2.Transform Agrobacterium with plasmid DNA | 3. Extract plasmid DNA from Agrobacterium |
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| 4. Tissue culture aseptic technique | 5. Tobacco leaf disc transformation | 6. Arabidopsis flower-dipping transformation |
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| 7. Maize transformation | 8. Plant DNA extraction | 9. Polymerase chain reaction (PCR) |
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| 10. Southrn blot and hydridization | ||
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Contact persons:
Kan Wang, 294-4429
Maximum enrollment: 12 students
12:30 - 4:00 pm, Wednesdays and Fridays
February 1, 3, 15, 17, 22, 24
March 29, 31
April 5, 12
