IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | OFFICE OF BIOTECHNOLOGY | PLANT SCIENCES INSTITUTE

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FACILITY
SERVICES	Maize Biolistic Transformation Maize Agro Transformation Soybean Transformation Rice Transformation Transformation Related Services
PRICING	Maize Biolistic Transformation Maize Agro Transformation Soybean Transformation Rice Transformation Transformation Related Services
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PERSONNEL	Director Monocot Team Soybean Team Greenhouse Team Molecular Analysis Team Graduate Student and Postdoc Team Previous Employees and Students Interns and Rotation Students Visiting Scientists
RESEARCH	Bio-pharmaceutical Bio-Renewable Resource Maize Inbred Transformation Maize Mature Seed Transformation Maize Male Sterile Transformation Nanoparticles Mediated Transformation Rice Transformation Soybean Half Seed Transformation Stress Tolerance Trafficking of Recombinant Proteins
TEACHING	2014 GDCB 542D course 2012 GDCB 542D course 2010 GDCB 542D course 2009 GDCB 542D course 2008 GDCB 542D course 2007 GDCB 542D course 2006 GDCB 542D course NSF Maize Transformation Workshop Interns and Rotation Students Visiting Scientists
SYMPOSIUMS	Workshop 2003 Workshop 2005 CINVESTAV
PUBLICATIONS	2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2004 2003 2002 2000 1999
PROTOCOLS	Maize Embryo Bombardment (pdf) Maize Callus Bombardment (pdf) Gold Particle Preparation (pdf) Maize Agrobacterium Transformation (pdf) Rice Transformation (pdf) Maize Greenhouse Care (pdf) Soybean protocol (pdf) Rice Growth Chamber Care (pdf) Plasmid DNA Miniprep from Agrobacterium (pdf)
LINKS
PTF Internal

Spring 2009 Workshop on Plant Transformation

GDCB 542D (1 credit)

Induction of crown gall tumor on tobacco plants

Tobacco transformation experiments

Maize and Rice transformation experiments

Maize and Rice results
Class photos: Biolistic Gun transformation, Maize and Rice Regeneration and Pipeline observations
Rice co-cultivation plate with Agrobacterium overgrowth
Maize Transformation Results Summary (May 5, 2009) (pdf 47KB)
Rice Transformation Results summary (May 5, 2009) (pdf 9KB)

Transgenic Plant Analysis

Plasmid DNA Digestion
PCR Analysis
Southern Analysis