Rice Genetic Transformation:
Agrobacterium-mediated
Nippon Bare rice seed - Starting material for callus initiation and transformation
The PTF Rice Transformation System:
Target tissue: Callus culture derived from mature embryo
Transformable cultivars: Japonica cv. Nipponbare, Kitaake
Delivery means: Agrobacterium tumefaciens
Selection: the hpt gene/hygromycin
Products available: Plantlets
Protocol: please see Rice Transformation Protocol
Time frame from infection to transgenic seed:
| Transformation Activity | Day |
|---|---|
| Initiate callus from mature seed | 1 |
| Select callus for transformation | 35 |
| Transform callus tissue | 39 |
| Pick and bulk bialaphos resistant callus events | 88-102 |
| Regenerate transgenic plantlets | 109-123 |
| Harvest transgenic seed | 220 |
