Spring Workshop on Plant Transformation and Transgenic Plant Analysis
GDCB 542D (1 credit)
Maize transformation experiments
Maize Transformation Results SummaryView your Maize regeneration photos
Tobacco Transformation experiments
Transgenic Plant Analysis
Agrobacterium DNAPCR analysis of transgenic plants
Southern analysis of transgenic plants
Objectives of the workshop:
This is a one-credit laboratory course aiming at learning basic knowledge and techniques on plant genetic transformation and molecular analysis of transgenic plants. Upon completion of the course, students should be able to do the following:
- Making plant media
- Introducing DNA vectors into Agrobacterium strains
- Plasmid DNA extraction from Agrobacterium
- Basic aseptic techniques for plant tissue culture
- Agrobacterium-mediated tobacco leaf disc transformation
- Arabidopsis flower-dipping transformation
- Some steps of the biolistic gun-mediated and Agrobacterium-mediated maize transformation
- Plant DNA extraction
- Polymerase Chain Reaction (PCR)
- Southern Blot and hybridization
- Graduate students majoring any discipline of plant sciences
- Graduate students majoring any science discipline but with an intention to collaborate with plant scientists.
- Any one who is interested in plant genetic transformation
Contact persons:
Kan Wang, 294-4429
Maximum enrollment: 12 students
Location: G217/B423-B426 Agronomy
12:30 - 4:00 pm, Wednesdays and Fridays
Total 10 classes:
February 7, 9, 21, 23, 28
March 2
April 4, 6, 11, 18
