IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | OFFICE OF BIOTECHNOLOGY | PLANT SCIENCES INSTITUTE

HOME
FACILITY
SERVICES	Maize Biolistic Transformation Maize Agro Transformation Soybean Transformation Rice Transformation Transformation Related Services
PRICING	Maize Biolistic Transformation Maize Agro Transformation Soybean Transformation Rice Transformation Transformation Related Services
Ordering Procedures
PERSONNEL	Director Monocot Team Soybean Team Greenhouse Team Molecular Analysis Team Graduate Student and Postdoc Team Previous Employees and Students Interns and Rotation Students Visiting Scientists
RESEARCH	Bio-pharmaceutical Bio-Renewable Resource Maize Inbred Transformation Maize Mature Seed Transformation Maize Male Sterile Transformation Nanoparticles Mediated Transformation Rice Transformation Soybean Half Seed Transformation Stress Tolerance Trafficking of Recombinant Proteins
TEACHING	2014 GDCB 542D course 2012 GDCB 542D course 2010 GDCB 542D course 2009 GDCB 542D course 2008 GDCB 542D course 2007 GDCB 542D course 2006 GDCB 542D course NSF Maize Transformation Workshop Interns and Rotation Students Visiting Scientists
SYMPOSIUMS	Workshop 2003 Workshop 2005 CINVESTAV
PUBLICATIONS	2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2004 2003 2002 2000 1999
PROTOCOLS	Maize Embryo Bombardment (pdf) Maize Callus Bombardment (pdf) Gold Particle Preparation (pdf) Maize Agrobacterium Transformation (pdf) Rice Transformation (pdf) Maize Greenhouse Care (pdf) Soybean protocol (pdf) Rice Growth Chamber Care (pdf) Plasmid DNA Miniprep from Agrobacterium (pdf)
LINKS
PTF Internal

Spring 2008 Workshop on Plant Transformation

GDCB 542D (1 credit)

Tobacco transformation experiments

Arabidopsis transformation experiments

Maize and Rice transformation experiments

Maize and Rice results from 2/27/08 regeneration
Class photos: Biolistic Gun transformation, Maize and Rice Regeneration and Pipeline observations on 2/27/08
Stable GUS expression in rice callus derived from "no-transient" pTF102 infected rice!
Maize Transformation Results Summary (April 23, 2008) (pdf 45KB)
Rice Transformation Results summary (April 23, 2008) (pdf 8.4KB)

Transgenic Plant Analysis

Binary vector DNA extraction from Agrobacterium
PCR analysis of transgenic plants
Southern analysis of transgenic plants