IOWA STATE UNIVERSITY | DEPARTMENT OF AGRONOMY | OFFICE OF BIOTECHNOLOGY | PLANT SCIENCES INSTITUTE

Service List


The ISU Plant Transformation Facility offers multiple services related to crop genetic transformation. We are constantly working to improve our overall productivity and to consistently meet the needs of our customers.


Maize Genetic Transformation: Biolistic-mediated

Biolistic transformation is a physical way to deliver exogenous DNA into a cell. We use the BioRad PDS/He 1000 device and 0.6 ┬Ám gold particles for maize transformation. Our most frequently used maize genotype is Hi II (a hybrid derived from A188 and B73).    More ...
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Maize Genetic Transformation: Agrobacterium-mediated

Agrobacterium tumefaciens is a soil bacterium that has the ability to transfer DNA into plant cells. We use disarmed Agrobacterium strains carrying standard binary vectors for transformation of immature embryos of maize Hi II genotype. Inbred maize (B104 genotype) is also offered using this protocol with sufficient lead time.    More ...
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Soybean Genetic Transformation

Agrobacterium-mediated transformation is used to transform this crop following a recently improved method that uses a half-seed explant from mature seed as the target tissue. Transformable soybean varieties include William 82 and Thorne.    More ...
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Rice Genetic Transformation

We use our expertise in Agrobacterium-mediated transformation to transform rice callus cultures. Two transformable Japonica varieties include Nipponbare and Kitaake.    More ...
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Transformation related services

Along with genetic transformation of crops our facility offers other transformation related services. You can purchase greenhouse grown maize ears, maize immature embryos, biolistic gun supplies and maize tissue culture media. In addition, we can introduce DNA construct into Agrobacterium strains for your transformation projects. Customers can even transform worms (C. elegans) using our biolistic gun.
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Consulting and Other

Our experienced staff is here to assist you at every stage of plant transformation from construct design to greenhouse care of transgenic plants. We also provide non-transgenic tissue or seed upon request that can be used either as an experimental control or for laboratory/greenhouse practices. Feel free to contact us with any questions regarding crop genetic transformation.
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NOTE: A cancellation fee will be charged for cancellation of any on-going work. The amount of the fee is proportional to the amount of work realized at the cancellation date.