Maize Genetic Transformation:
Agrobacterium-mediated
PTF Maize Transformation System:
Method optimized in PTF lab (Frame et al. 2002)
Target tissue: Immature zygotic embryo
Transformable genotypes: Hi Type II hybrid
Delivery means: Agrobacterium tumefaciens
Selection: the bar gene/bialaphos
Products Available: Plantlets, seed, callus
Protocol: please see Maize Agrobacterium Transformation Protocol
Bialaphos resistant event regenerating to produce plants
Days from infection to transgenic seed shipment:
| Transformation Activity | Hi II | B104 |
|---|---|---|
| Embryo infection | 1 | 1 |
| Pick and bulk bialaphos resistant callus events | 80 | 110 |
| Regenerate transgenic plantlets to soil | 120 | 140 |
| Ship transgenic seed | 250 | 270 |
