Soybean Genetic Transformation:
Agrobacterium-mediated
The PTF Soybean Transformation System:
Method optimized in PTF lab (Paz et al. 2004)
Target tissue: Half-seed explants
Transformable cultivars: Thorne, Williams, Williams 82
Delivery means: Agrobacterium tumefaciens
Selection: the bar gene/bialaphos
Products available: seed
Protocol: please see Soybean Transformation Protocol
Bialaphos resistant soybean plantlet on regeneration medium
Time frame from infection to transgenic seeds:
| Transformation Activity | Day |
|---|---|
| Explant dissection and infection | 1 |
| Selection and shoot regeneration of bialaphos resistant events | 6 (8-12 wks) |
| Rooting | 56 |
| Plants to soil | 70 |
| Harvest | 240 |
| Progeny analysis and delivery | 270 |
