Maize Genetic Transformation:
Biolistic-mediated
The PTF Maize Transformation System:
Method optimized in PTF lab (Frame et al. 2000)
Target tissue: Immature zygotic embryos or callus cultures derived from immature embryo.
Transformable genotypes: Hi Type II hybrid;
Delivery means: BioRad PDS 1000/He biolistic gun
Selection: the bar gene/bialaphos
Products available: Callus, plantlets, seed
Protocol: please see Maize Embryo Bombardment Protocol or Maize Callus Bombardment Protocol
Days from bombardment to transgenic seed delivery:
Transformation Activity | Hi II |
---|---|
Embryo bombardment | 1 |
Pick and bulk bialaphos resistant callus events | 80 |
Confirm co-transformation | 100 |
Regenerate transgenic plantlets to soil | 140 |
Ship transgenic seed | 260 |